The mass spectrometry-based Proteomics Resource Facility (PRF) at the Department of Pathology began its operations in 2007 and the facility occupies about 1500 sq feet of space in room 3554/3556 at MSRB2.
The goal of the PRF is to assist researchers in designing proteomic experiment, maintain state-of-the-art instrumentation, provide technical expertise and bioinformatic support during both discovery and validation phases of proteomic experiments.
During the discovery phase, the services offered by the PRF include identification of proteins from multiple sources, such as Coomassie and Silver-stained gels, identification of post-translational modifications, and shot-gun proteomic analysis of complex mixtures using either one- or two-dimensional liquid chromatography (2D-LC). Relative quantitation (differential proteomic analysis) using stable isotope labeling of amino acids in culture (SILAC), isotope-coded affinity tags (eg. TMT) and label-free spectral counting are also offered.
For validation of findings from the discovery phase (especially projects related to biomarker discovery), a researcher may wish to employ a more targeted approach and expand the sample size. The PRF provides support for relative and absolute quantification (AQUA) of multiple analytes using Parallel Reaction Monitoring (PRM).
The PRF is equipped with two Thermo Scientific mass spectrometers devoted exclusively for proteomic analysis. These include
1. Orbitrap Fusion Tribrid with ETD
2. Q Exactive HF
Both mass spectrometers are equipped with dedicated nano-LC systems (Dionex RSLC-nano).
Acknowledgment of Contributions
Publications containing data generated by the Proteomics Resource Facility (PRF) should include an acknowledgement of the PRF or include members as coauthors. The PRF needs these acknowledgements and authorship to help demonstrate our contributions to the research community. This in turn helps secure future funding to maintain a robust Core facility and provide professional development of its staff. The Association of Biomolecular Resource Facilities (ABRF) has published a guideline to use when considering whether or not to include core laboratory members on publications.